PP PE WPC Series Profile Extrusion Line PP Series Profile Extrusion Line,PE Series Profile Extrusion Line,WPC Series Profile Extrusion Line zhangjiagang sevenstars machinery co.,ltd , https://www.sevenstarmachienry.com
Jujube tissue culture seedlings generally adopt MS full salt culture in the proliferation stage, while 1/2MS or H medium is used in the rooting process, and the nitrate content of these two kinds of culture media is low; while in the northern loessial soil of northern Shaanxi region The nitro content is even lower than in the medium. Jujube test tube seedlings after rooting generally after hardening seedling transplant can enter the greenhouse first, and then transferred to the field. The branches of Daejeon Miao can be directly inserted into Daejeon. There is a big difference between the two. In this paper, we studied the difference of chlorophyll and chlorophyll content between jujube tree seedlings (subculture and rooting) chlorophyll and field seedlings, in order to provide a basis for the study of artificial breeding and natural breeding of jujube tree on the difference of jujube life ability.
Randomly selected 25 days from MS growth medium, the heads of the seedlings of Ziziphus jujuba, Ziziphus jujuba, and Zizyphus jujube in the MS medium were randomly selected and cultured for 25 days in the 1/2MS medium. Jujube hanging relative position close to the dog head jujube, jujube, jujube leaves each 50. Each leaf was randomly selected 6 points and the relative amount of chlorophyll was measured with a SPAD-502 portable chlorophyll meter. Measurement of test-tube seedlings Before the daylight lamp was turned on, measurements of Daejeon seedlings were conducted before sunrise in the morning. After the measurement of the living body, the total chlorophyll content was measured by the acetone-ethanol extraction method. Data processing is based on statistical principles and analyzed using Excel 2003 software.
The chlorophyll content in the woody jujube measured by the portable chlorophyll meter SPAD-502 method varies from 3.57 to 8.37 m"dm-z from the subculture seedlings to the field seedlings. The Daejeon seedling has the highest chlorophyll content and the average value is 7.17m. }"dm-z, the smallest coefficient of variation, 0.065; sub-seedlings had the lowest chlorophyll content, with an average of 4.23 m}"dm-z, with a maximum coefficient of variation of 0.089. The jujube chlorophyll was determined by the acetone-ethanol extraction method. The contents varied from 1.564.02 m.The chlorophyll content of the Datian seedlings was the highest with an average of 3.56 m; the chlorophyll content of the seedlings in the test tube was the lowest with an average of 1.79 m, and the variation coefficient of the secondary seedling chlorophyll content was the smallest, being 0.037. The largest seedling of Daejeon was 0.061.
The chlorophyll content of all three jujube tree species from the secondary seedlings of the test tube to the field seedlings was measured by the portable chlorophyll meter and was found to vary from low to high. In addition, the chlorophyll content measured by the two methods of wood jujube and jujube in Datian seedlings was higher than that of dog head jujube. This phenomenon may be related to the adaptability of the jujube trees of these three species. Jin jujube and jujube were distributed in Shaanxi and Shanxi, while the dog head jujube only had a small amount in Yanchuan, Shaanxi.
Analysis of chlorophyll content of jujube cultivars in vitro by portable chlorophyll meter
Chlorophyll is a pigment that is required for plant survival, and has a very close relationship with photosynthesis. The portable chlorophyll meter can determine the relative content of chlorophyll in plant leaves through the greenness of the plant, so as to understand the true nitro requirement of the plant and help to understand the degree of nitro deficiency in the soil and determine whether excessive nitrogen fertilizer is applied.